1. Ennyonyola ya sayansi n’ensibuko y’ebyafaayo by’ekikuta kya citrus aurantium .
Citrus Aurantium Extract ye kibinja ky’ebitundu ebikola ebifunibwa okuva mu .Ebibala ebikalu ebikalu .wa citrus aurantium L. oba citrus sinensis Osbeck (amaka ga RUTACEAE) nga bayita mu bukodyo obw’omulembe obw’okuggyamu. Enkozesa yaayo ey’eddagala edda ku:
"Shennong Ben Cao Jing".(Eastern Han Dynasty): Ekigabanyizibwamu nga wakati-omuddo ogw'ekika, oguwandiikiddwa "okujjanjaba empewo ey'amaanyi mu lususu n'okugonjoola ennyonta-okusigala kw'ebbugumu."
"Shang Han Lun".(Zhang Zhongjing): Ekozesebwa ng'omuddo omufuzi mu nsengekera za kikula nga "Da Cheng Qi Tang."
Omutindo gwa Pharmacopoeia ogw'omulembe .: Enkola ya 2020 eya China Pharmacopoeia eraga ekitono ennyo hesperidin ekiri 4.0%.
2. Okwekenenya okutegekeddwa kw’ebitundu ebikulu .
2.1 Ebirungo ebiyitibwa alkaloids .
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Ekitundu . |
Ebirimu range . |
ekigendererwa ky’ekitundu ky’omubiri . |
Ekikolwa ky’eddagala . |
|
Synephrine . |
0.3-1.2% |
Ebitundu by’omubiri ebizimba omubiri . |
Akola mu ngeri ey’okulondamu ebikwata 3-AR, okutumbula lipolysis (EC50=3.2μm) . |
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N -EKITABO KYA METHYLTYRAMINE . |
0.08-0.15% |
GI Ekinywa ekiweweevu . |
Yanguyira okufulumya amazzi mu lubuto ng’eyita mu 5-HT4 receptors (1.8x amaanyi okusinga domperidone) |
2.2 Ebirungo ebiyitibwa flavonoids .
EkirungoHesperIdinneohesPerIdinnaringinnobiletin .
Hesperidin .: Eziyiza ekyenda -glucosidase (IC 50=28μm) .
Nobiletin .: Ekola AMPK-Ekkubo lya SIRT1-PGC1 (Eyongereza ku mitochondrial biogenesis) .
2.3 Amafuta agawunya .
D -limonene (32.6%): esika okufulumya kw’entuuyo (dose -esinziira ku)
-Terpinene (18.4%): Eziyiza okukula kwa H. pylori (MIC=62.5ug/ml)
3. Multi-Enkola z’okukola ez’ebitundutundu .
3.1 Okulungamya enkola y’okugaaya emmere .
Ennyongera ku ntambula y’omu lubuto .:
Yongera ku bunene bw’okukonziba kw’omu maaso (+40%) ng’ayita mu kufulumya obusimu obutambuza obusimu obuyitibwa cholinergic neurotransmitter .
Akendeeza ku kitundu ky'omu lubuto-Ekiseera ky'okugonza (okuva ku 45min→28min)
Gut microbiota modulation .:
Eyongeza okubala kwa bifidobacterium/Lactobacillus (3-5x) .
Ekendeeza ku mugerageranyo gwa E. coli (↓60%) .
3.2 Omukutu gw’okulungamya enkyukakyuka mu mubiri .
4. Enkola y’okulondoola omutindo .
4.1 Okufuga ebintu ebisookerwako .
Okukungula obudde: Obuwanvu bw’ebibala 1.5-2cm (peak flavonoid ebirimu)
Ensibuko y’ensi: Jiangxi (Ekitundu Ekituufu ekivaamu) Efulumya ebitundu 30% ebisinga obungi byonna ebiva mu flavonoids .
4.2 Enkola y’okufulumya .
Pre-Okujjanjaba .: Okubetenta wansi-kutono ({0}}.<40°C)
Okuggyamu .: Okuggya ethanol mu ddaala (50%→70%)
Okulongoosa .: Okusikiriza kwa macroporous resin (AB-8 type) .
4.3 Ebipimo bya QC .
Okufaanagana kw’engalo za chromatographic okusinga oba okwenkana 0.95 .
Ebyuma Ebizito: PB .<5ppm, As <2ppm
Eddagala eritta ebiwuka: BHC .<0.1mg/kg
5. Ebikwata ku kunoonyereza ku bujjanjabi .
5.1 Obulwadde bw’okutabulwa mu nkola (RCT) .
Design: emirundi ebiri-efugirwa ekizibe (n=240) .
Alizaati:
Obubonero bw’obubonero: 82.5% vs control 58.3% (P .<0.01)
Electrogastrogram: okulongoosa ennyimba oluvannyuma lw'okulya +35% .
5.2 Obulwadde bw’okukyusakyusa emmere (meta-Okwekeneenya) .
Okunoonyereza 12 kwalimu (emisango 1,842) .
Ebivaamu:
Okwetooloola ekiwato: -3.8cm (95%CI:-4.2~-3.4)
Glucose mu kusiiba: -0.82mmol/l (p=0.003) .
6. Okukozesa obulungi protoc .OLS .
6.1 Dozi-Enkolagana y’okuddamu .
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Ekiraga . |
Dozi esengekeddwa . |
Peak Effect obudde . |
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Obujjuvu bw'oluvannyuma lw'okulya . |
200mg tid . |
30min . |
|
Okuddukanya obuzito . |
500mg + EGCG Combo . |
Wiiki 8 . |
6.2 Okugatta okukontana .
Okwegendereza ne:
Ephedrine-Eddagala eririmu (ziyinza okwongera ku bikolwa by'emisuwa n'emitima) .
Anticholinergics (ayinza okukendeeza ku bulung’amu) .
6.3 Abantu ab’enjawulo .
Abakadde: Tandika ku 50% standard dose
Okufuna olubuto: Okuziyiza (ayinza okusitula okukonziba kwa nnabaana)
7. Ekitabo ekikwata ku kwekenneenya ebintu mu katale .
7.1 Ebintu ebikozesebwa mu kukola ebintu eby’omutindo ogwa waggulu .
Ewandiikiddwako "Ekitongole ekituufu-Ensibuko y'ebimera" .
Awa engalo za HPLC .
GMP-Eratibwa .
7.2 Enkola za bulijjo ez’okwetaba .
Yayongeddeko Synthetic Synephrine .
admixture ne tangerine peel extract .
8. Okusala-Endagiriro z’okunoonyereza ku .
Gut-Ekikondo ky’obwongo: Ebikosa ebikwata ku 5-HT ebikwata .
Obusobozi bw'okulwanyisa ekizimba: Wnt/ -Okuziyiza ekkubo lya catenin .
Enkola z’okutuusa ebintu ku novel: nanoemulsions for enhanced bioavailability .
Mu bufunzi
Citrus Aurantium extract eyimiridde nga ekitereeza eky’obutonde ekitasobola kukyusibwa mu kugaaya emmere-obulamu bwa metabolic okuyita mu:
1️⃣ Multi-Okukwatagana kw'ekitundu .(alkaloid -Flavonoid-Obusatu obuwunya amafuta) .
2️⃣ Ebikolwa ebingi-Target .(Ekigaaya -Ekitundu ky'omubiri (metabolic)-Okukyusakyusa mu buziyiza) .
3️⃣ Okugaba dose mu butuufu .(enkola ezikakasibwa mu ngeri y’ekiwandiiko) .
Okusobola okukola obulungi, ebintu ebirondeddwamu ebikoleddwa okuva mu .Ebintu ebituufu GEO-Ebikozesebwa mu kuggya ., era ogoberere ebiragiro by’okukozesa ebikukwatako .
